Plasmid

Part:BBa_I718020:Design

Designed by: Eimad Shotar   Group: iGEM07_Paris   (2007-10-26)


ptet-lox71-rbs-gfp-ter-lox66-mRFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1048
    Illegal PstI site found at 1931
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1048
    Illegal PstI site found at 1931
    Illegal NotI site found at 7
    Illegal NotI site found at 1924
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1048
    Illegal PstI site found at 1931
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1048
    Illegal PstI site found at 1931
    Illegal AgeI site found at 1627
    Illegal AgeI site found at 1739
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 791
    Illegal BsaI.rc site found at 3012


Design Notes

none


Source

none

References